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canine adipocyte differentiation medium  (Cell Applications Inc)


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    Structured Review

    Cell Applications Inc canine adipocyte differentiation medium
    Canine Adipocyte Differentiation Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adipocyte+differentiation+medium/Canine+Adipocyte+Differentiation+Medium/pmc12159829-82-5-9
    Average 94 stars, based on 8 article reviews
    canine adipocyte differentiation medium - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cell Differentiation:

    Article Title: Structure and function of modular microfluidic device for in-vitro modeling of human organs
    Article Snippet: .. To induce cell differentiation, the preadipocyte growth medium was replaced with an adipocyte differentiation medium (#811D-250, Cell Applications). ..

    other:

    Article Title: Impact of housing temperature on adipose tissue HDAC9 expression and adipogenic differentiation in high fat-fed mice.
    Article Snippet: Department of Medicine, Medical College of Georgia at Augusta University, Augusta, Georgia, USA Vascular Biology Center, Medical College of Georgia at Augusta University, Augusta, Georgia, USA Department of Pharmacology and Toxicology, Medical College of Georgia at Augusta University, Augusta, Georgia, USA Department of Surgery, Medical College of Georgia at Augusta University, Augusta, Georgia, USA Department of Neuroscience and Regenerative Medicine, Medical College of Georgia at Augusta University, Augusta, Georgia, USA Department of Physiology, Medical College of Georgia at Augusta University, Augusta, Georgia, USA

    Cell Culture:

    Article Title: Pharmacological antagonism of receptor for advanced glycation end products signaling promotes thermogenesis, healthful body mass and composition, and metabolism in mice.
    Article Snippet: Human subcutaneous primary adipocytes (deidentified White female, BMI = 20.6, age = 34 years) were procured from Cell Applications (S802s-05a, Lot#1687). .. Preadipocytes were cultured in preadipocyte growth medium (Cell Applications 811-500) until 100% confluence and differentiated to adipocytes in adipocyte differentiation medium (Cell Applications 811D-250) for 12 to 13 days. ..

    Article Title: Intra-articular injection of adipose-derived mesenchymal stem cell line attenuates pain, synovitis and cartilage degeneration in monoiodoacetate-induced osteoarthritis rat model
    Article Snippet: .. Brie y, ADSCs were cultured with Adipocyte Differentiation Medium (Cell Applications Inc.), and fat drops from trypsinized cells underwent upside-down culture. ..

    Article Title: Cationic polymer effect on brown adipogenic induction of dedifferentiated fat cells
    Article Snippet: .. To obtain WAT drops, samples were cultured in Adipocyte Differentiation Medium (Cell Applications Inc.) for 2 weeks with half the medium replaced every two days throughout the culturing period. ..

    Modification:

    Article Title: Structure and function of modular microfluidic device for in-vitro modeling of human organs
    Article Snippet: Poly-L-lysine (PLL) solution, 0.1% (w/v), collagen (type 1, rat tail) and Trypsin/EDTA were purchased from Sigma Aldrich (Burlington, Massachusetts, USA). .. Dulbecco's Modified Eagle Medium/Ham's F-12 (DMEM/F12) containing 10% (v/v) fetal bovine serum and 1% penicillin-streptomycin antibiotic was purchased from Gibco., human pre-adipocytes (HPAd) cat #802s-05a, pre-adipocyte growth medium, adipocyte differentiation medium, adipocyte maintenance medium, and adipocyte starvation medium from Cell Applications (USA); iMDM medium from Thermo Fisher Scientific (USA); Lipid A (LPA) from E. coli serotype R515 (TLR grade) from Alexis Biochemical (SA, USA); TNF-α, IL-6 and IL-8 ELISA kits, CD11b-FITC (clone ICRF44), CD45-PE (clone: 2D1), CD3-PerCP-Cy5.5 (cline: HIT3A), CD19-PE-Cy7 (clone: calceine-AM from ThermoFisher Scientific (USA); 2-NBDG glucose uptake cell-based assay kit from Cayman Chemical (USA); Hoechst 33342 from Molecular Probe (OR, USA); Bradford reagent and 0.4% trypan blue, metformin hydrochloride and docosapentaenoic acid (DPA) from Sigma Aldrich (USA); Other chemicals were of analytical grades from Sigma Aldrich (USA). .. Fabrication: The microfluidic systems can be fabricated using a variety of micro fabrication techniques including silicon micromachining, soft lithography, injection molding, 3D printing and laser machining.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Structure and function of modular microfluidic device for in-vitro modeling of human organs
    Article Snippet: Poly-L-lysine (PLL) solution, 0.1% (w/v), collagen (type 1, rat tail) and Trypsin/EDTA were purchased from Sigma Aldrich (Burlington, Massachusetts, USA). .. Dulbecco's Modified Eagle Medium/Ham's F-12 (DMEM/F12) containing 10% (v/v) fetal bovine serum and 1% penicillin-streptomycin antibiotic was purchased from Gibco., human pre-adipocytes (HPAd) cat #802s-05a, pre-adipocyte growth medium, adipocyte differentiation medium, adipocyte maintenance medium, and adipocyte starvation medium from Cell Applications (USA); iMDM medium from Thermo Fisher Scientific (USA); Lipid A (LPA) from E. coli serotype R515 (TLR grade) from Alexis Biochemical (SA, USA); TNF-α, IL-6 and IL-8 ELISA kits, CD11b-FITC (clone ICRF44), CD45-PE (clone: 2D1), CD3-PerCP-Cy5.5 (cline: HIT3A), CD19-PE-Cy7 (clone: calceine-AM from ThermoFisher Scientific (USA); 2-NBDG glucose uptake cell-based assay kit from Cayman Chemical (USA); Hoechst 33342 from Molecular Probe (OR, USA); Bradford reagent and 0.4% trypan blue, metformin hydrochloride and docosapentaenoic acid (DPA) from Sigma Aldrich (USA); Other chemicals were of analytical grades from Sigma Aldrich (USA). .. Fabrication: The microfluidic systems can be fabricated using a variety of micro fabrication techniques including silicon micromachining, soft lithography, injection molding, 3D printing and laser machining.

    Cell Based Assay:

    Article Title: Structure and function of modular microfluidic device for in-vitro modeling of human organs
    Article Snippet: Poly-L-lysine (PLL) solution, 0.1% (w/v), collagen (type 1, rat tail) and Trypsin/EDTA were purchased from Sigma Aldrich (Burlington, Massachusetts, USA). .. Dulbecco's Modified Eagle Medium/Ham's F-12 (DMEM/F12) containing 10% (v/v) fetal bovine serum and 1% penicillin-streptomycin antibiotic was purchased from Gibco., human pre-adipocytes (HPAd) cat #802s-05a, pre-adipocyte growth medium, adipocyte differentiation medium, adipocyte maintenance medium, and adipocyte starvation medium from Cell Applications (USA); iMDM medium from Thermo Fisher Scientific (USA); Lipid A (LPA) from E. coli serotype R515 (TLR grade) from Alexis Biochemical (SA, USA); TNF-α, IL-6 and IL-8 ELISA kits, CD11b-FITC (clone ICRF44), CD45-PE (clone: 2D1), CD3-PerCP-Cy5.5 (cline: HIT3A), CD19-PE-Cy7 (clone: calceine-AM from ThermoFisher Scientific (USA); 2-NBDG glucose uptake cell-based assay kit from Cayman Chemical (USA); Hoechst 33342 from Molecular Probe (OR, USA); Bradford reagent and 0.4% trypan blue, metformin hydrochloride and docosapentaenoic acid (DPA) from Sigma Aldrich (USA); Other chemicals were of analytical grades from Sigma Aldrich (USA). .. Fabrication: The microfluidic systems can be fabricated using a variety of micro fabrication techniques including silicon micromachining, soft lithography, injection molding, 3D printing and laser machining.



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    Image Search Results


    Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 (Mesenchymal cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the

    Journal: Regenerative Therapy

    Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

    doi: 10.1016/j.reth.2025.101056

    Figure Lengend Snippet: Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 (Mesenchymal cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the "Chondrocyte" cluster, and Col1a1 for the "Mesenchymal cell" cluster, and CD45/CD14 for the "Hematopoietic" cluster.

    Article Snippet: For adipocyte differentiation, either BioMirai Lab's induction kit or PromoCell's mesenchymal stem cell adipocyte differentiation medium was used, with Oil Red O staining to assess lipid accumulation.

    Techniques: RNA Sequencing, Gene Expression, Clinical Proteomics, Expressing, Marker

    Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

    Journal: Regenerative Therapy

    Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

    doi: 10.1016/j.reth.2025.101056

    Figure Lengend Snippet: Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

    Article Snippet: For adipocyte differentiation, either BioMirai Lab's induction kit or PromoCell's mesenchymal stem cell adipocyte differentiation medium was used, with Oil Red O staining to assess lipid accumulation.

    Techniques: Gene Expression, RNA Sequencing, Comparison, Flow Cytometry